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EDTA Blood Collection Tube for Liquid Biopsy with Cell Protection

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Shanghai Orsin Medical Technology Co., Ltd.

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EDTA Blood Collection Tube for Liquid Biopsy with Cell Protection

Name: EDTA Blood Collection Tube for Liquid Biopsy with Cell Protection
Brand: Orsin
Price: 1 USD

Wholesale EDTA Blood Collection Tube for Liquid Biopsy with Cell Protection from china suppliers

Brand Name : Orsin

Model Number : Gel Clot ActivatoR

Certification : ISO 13485

Place of Origin : China

MOQ : 100/pcs

Price : Negotiation

Payment Terms : L/C,D/A,D/P,T/T,Western Union

Supply Ability : 20million/pcs/moth

Delivery Time : 1-4 weeks

Tube Type : EDTA

Application Scenarios : Liquid Biopsy

Tube Cap : Plastic

Storage Condition : Room Temperature

Product Nam : Cell Free DNA

Superiority : Cell Protection

Key Features : Rapid clotting

Additive : Sodium Citrate/Sodium Heparin/EDTA

Sterilization Method : Gamma Radiation/Ethylene Oxide

Closure Type : Screw Cap/Rubber Stopper

Characteristic : Excellent stability

Behavior : Strong temperature tolerance

Advantages : High recovery yield

Logo Printing : Available for big orders (>30000 PCS)

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I. Product FeaturesPrecision Anticoagulation Mechanism
Utilizes potassium ethylenediaminetetraacetate (EDTA-K₂ or EDTA-K₃) to inhibit coagulation by chelating calcium ions, preserving blood cell morphology and quantitative integrity.
Strict anticoagulant dosage of 1.5–2.2 mg/mL blood, ensuring optimal anticoagulation without under- or over-treatment.
Premium Materials & Craftsmanship
Tube Material: High-clarity medical-grade PET plastic (lightweight, shatter-resistant) or glass (for large volumes), with silicone-coated interior to prevent cell adhesion and hemolysis.
Sealing System: Butyl rubber stopper offers superior airtightness (>40,000 puncture tolerance), minimizing needle wear.
Vacuum Accuracy: Imported equipment guarantees blood draw volume error ≤±5% (e.g., 1.9–2.1 mL for 2 mL tubes).
Standardization & Safety
Purple cap (universal color code) for rapid identification.
Gamma-irradiated sterilization, endotoxin-compliant, certified to ISO 13485 and CE standards.
II. Application Scenarios

	
			Test Type
			Specific Applications
			Mechanism
		

	
			Basic Hematology
			Complete Blood Count (CBC), blood typing, erythrocyte sedimentation rate (requires dedicated tube)
			Preserves cell morphology, prevents clotting interference
		

			Specialized Tests
			HbA1c, flow cytometry, pathogen nucleic acid testing (PCR)
			Stabilizes RBC membranes, protects DNA/RNA integrity
		

			Contraindications
			Coagulation tests (PT/APTT), calcium/potassium ion assays, trace element analysis
			EDTA chelation disrupts ion balance and clotting factor activity
		

III. Key Specifications (Table)

Parameter	K2/K3 EDTA Tube Specifications	Remarks
Volume Options	1-10ml (PET/GLASS)	Accommodates diverse sample volume needs
Anticoagulant Conc.	1.5–2.2 mg/mL blood	<1.5 mg/mL risks clotting; >2.2 mg/mL shrinks cells
Centrifugation	3500–4000 rpm × 5 min	Separates plasma/cellular layers

IV. Precautions

Collection Protocol
- Gently invert tubes 5–10 times immediately after draw—do not shake—to prevent microclots.
- Fill to marked volume: underfilling causes partial clotting; overfilling dilutes anticoagulant (causing pseudothrombocytopenia).
Sample Handling & Storage
- Time Sensitivity: Prepare blood smears within 2 hours; whole blood stored ≤6 hrs (RT) or ≤4 hrs (4°C)—prolonged storage alters cell morphology.
- Temperature: Ideal testing range: 18–22°C; extremes (>30°C or <15°C) affect cell volume.
Order of Draw
Follow sequence: Blood culture → Plain tubes → Citrate coagulation tubes → EDTA tubes to avoid cross-contamination.
V. Frequently Asked Questions (FAQs)
Microclots observed post-collection. Solution?
Cause: Inadequate/untimely mixing (especially at >25°C) or underfilled tube.
Action: Invert ≥8 times; ensure precise fill volume.
EDTA-K₂ vs. EDTA-K₃: Key differences?
- Form: K₂ = crystals; K₃ = liquid spray (faster dissolution).
- Impact: K₃ overuse dilutes samples (falsely lowers Hb); K₂ offers better batch consistency.
Suitable for coagulation tests?
No! EDTA chelates calcium, disrupting coagulation cascades. Use dedicated citrate tubes (blue cap) instead.
Causes/prevention of hemolysis?
Causes: Blood impacting tube bottom, violent mixing, premature refrigeration (<30 min post-draw).
Prevention: Angle tubes to let blood flow along walls; use large-bore needles; invert gently.
Preventing blood reflux infection?
Procedure: After draw → Remove tube first → Release tourniquet → Withdraw needle. Prevents negative-pressure backflow.

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